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《INT J MOL SCI》关于植物提取物在抗痤疮制剂中的应用研究

更新日期:2017年12月08日

1. 植物提取物治疗痤疮的抗菌和抗氧化方法

 

摘要:“寻常痤疮”是一种慢性皮肤感染,对大多数印度人都有影响。造成这种感染的内部和外部因素有很多。本文着重于筛选可对抗引起痤疮的细菌的药用植物提取物,并研究这些植物提取物的抗氧化活性。采用琼脂扩散法对迷迭香,天竺葵,罗勒,金合欢,印度楝,洋甘菊和金盏菊等的甲醇提取物进行抗菌活性分析,主要研究对大肠杆菌,铜绿假单胞菌,金黄色葡萄球菌,枯草芽孢杆菌和痤疮丙酸杆菌的影响。结果表明,不同的植物提取物对不同的测试样品有明显的活性差异。迷迭香(R. officinalis),洋甘菊(M. chamomilla)和金合欢(A. nilotica)的植物提取物对直径分别为8mm,6mm和4mm的痤疮丙酸杆菌具有显着的活性。我们对这三种植物提取物的最小抑制浓度(MIC)进行了测定。将迷迭香和洋甘菊提取物的MIC值与标准参考药物异维甲酸相比。由于抗氧化剂在痤疮的病理生理学中也起着重要的作用,因此使用二苯代苦味基自由基(DPPH)法对植物提取物进行抗氧化活性研究。用抗坏血酸作为抗氧化活性测定的标准。迷迭香,洋甘菊和金合欢的植物提取物具有中度抗氧化活性,IC50值分别109.3,136.9和108μg/ml。研究结果表明,迷迭香,洋甘菊和金合欢的植物提取物可以用作天然抗痤疮制剂。


Antibacterial and antioxidant strategies for acne treatment through plant extracts(原文)

 

Abstract: Acne vulgaris is a chronic skin infection affecting the majority of Indian population. There are several internal and external factors responsible for this infection. The present study emphasizes on screening of medicinal plant extracts against acne causing bacteria and antioxidant activity of these plant extracts. Anti-bacterial activities of methanolic extracts of Rosmarinus officinalis, Pelargonium asperum, Ocimum tenuiflorum, Acacia nilotica, Azadirachta indica, Matricaria chamomilla and Calendula officinalis was carried out using agar well diffusion method against Escherichia coli, Pseudomonas aeruginosa, Staphylococcus aureus, Kocuria sp., Bacillus subtilis and Propionibacterium acnes. The results revealed that different plant extracts showed noticeable activity against different test organisms.The plant extracts of R. officinalis, M. chamomilla and A. nilotica showed significant activity against P. acnes with diameter of 8 mm, 6 mm and 4 mm inhibition zone respectively. The minimum inhibition concentration (MIC)was measured for these three potential plant extracts. The MIC values of extracts of R. officinalis, M. chamomilla were comparable to the standard reference drugs isotretinoin. Since antioxidants also play an important role in the pathophysiology of acne, anti-oxidant activity of plant extracts was also carried out using 1, 1-diphenyl-2-picryl hydrazyl (DPPH) method. Ascorbic acid was used as a standard in anti-oxidant activity assay. The extracts of R. officinalis, A. nilotica and A. indica also exhibited moderate antioxidant activity with IC50 value of 109.3,136.9 and 108 μg/ml, respectively. The outcome of the study suggested that R. officinalis, M. chamomilla and A. nilotica plant extracts could be possible to use as the natural anti-acne formulations.




2.基于植物源抗菌抗炎化合物的新型抗痤疮制剂的研制与序列分析

摘要:天然植物衍生化合物的抗菌和抗炎潜力已经在许多研究中被报道。最新研究表明,从植物中提取的精油和其主要化合物可能是治疗青春期和成年人普遍存在的皮肤疾病——痤疮的可行的替代疗法。因此,本研究的目的是开发并分析一种基于植物衍生的抗菌化合物(即丁香酚,β-蒎烯,桉油精和柠檬烯)和抗炎剂的新型协同制剂,可用于局部治疗痤疮和其他皮肤感染。本研究选择的最佳抗菌组合为丁香酚/β-蒎烯/水杨酸和丁香酚/β-蒎烯/ 2-苯氧乙醇/山梨酸钾。流式细胞术揭示的可能的作用机制是细胞透化作用和抑制外排泵活性,其中外排泵活性可由对应于亚最小抑制(sub-MIC)值的浓度体现。以水杨酸/丁香酚/β-蒎烯/ 2-苯氧乙醇/山梨酸钾为代表的最有效的抗菌组合包裹于乳膏基质中,表现出热力学稳定性和最佳微生物学特性。


Development and Sequential Analysis of a New Multi-Agent, Anti-Acne Formulation Based on Plant-Derived Antimicrobial and Anti-Inflammatory Compounds(原文)

 

Abstract: The antibacterial and anti-inflammatory potential of natural, plant-derived compounds has been reported in many studies. Emerging evidence indicates that plant-derived essential oils and/or their major compounds may represent a plausible alternative treatment for acne, aprevalent skin disorder in both adolescent and adult populations. Therefore, the purpose of this study was to develop and subsequently analyze the antimicrobial activity of a new multi-agent, synergic formulation based on plant-derived antimicrobial compounds (i.e., eugenol, β-pinene, eucalyptol, and limonene) and anti-inflammatory agents for potential use in the topical treatment of acne and other skin infections. The optimal antimicrobial combinations selected in this study were eugenol/β-pinene/salicylic acid and eugenol/β-pinene/2-phenoxyethanol/potassium sorbate.The possible mechanisms of action revealed by flow cytometry were cellular permeabilization and inhibition of efflux pumps activity induced by concentrations corresponding to sub-minimal inhibitory (sub-MIC) values. The most active antimicrobial combination represented by salycilic acid/eugenol/β-pinene/2-phenoxyethanol/potassium sorbate was included in a cream base, which demonstrated thermodynamic stability and optimum microbiological characteristics.



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