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红枫叶提取物中含有葡萄糖醇核的没食子丹宁在化妆品中的应用

更新日期:2017年05月05日


研究富含酚醛树脂的红枫叶提取物中含有葡萄糖醇核的没食子丹宁在化妆品中的应用:通过下调B16F10黑色素瘤细胞中的酪氨酸酶和黑色素基因表达抑制黑色素原的生成


Cosmetic applications of glucitol-core containing gallotannins from a proprietary phenolic-enriched red maple (Acer rubrum) leaves extract: inhibition of melanogenesis via down-regulation of tyrosinase and melanogenic gene expression in B16F10 melanoma cells.



摘要:

     红枫(美国红枫)富含酚类物质,这些物质含有没食子酰基,附着在1,5-脱水葡萄糖醇核的不同位置。已有报告显示这些含有葡萄糖醇核的没食子丹宁(GCGs)具有抗氧化性和抗糖化性,但其在美妆领域的应用还未得到评价。本文通过使用酶和细胞检测法对专有的富含酚醛树脂的红枫叶提取物(Maplifa:含钙45%茶条槭素(GA)以及其他GCGs))的抑制酪氨酸酶和抗黑色素原特性进行调查。在IC50值为101.4-1047.3μm之间时,GCGs显示抑制酪氨酸酶。通过螯合及计算/建模研究对抑制酪氨酸酶的机制(将GA作为GCG的代表)进行了评估。GA使鼠黑色素瘤B16F10细胞中的黑色素含量降低了79.1,降低了56.7%(分别在浓度为25和50μm的无毒聚合物中)。通过荧光探针(DCF-DA)、实时聚合酶链反应和蛋白免疫印记试验对其抗黑色素原生成的机制进行了评估。数据显示GA能够做到以下几项:(1)降低活性氧水平,(2)通过时间依赖方式下调MITF,TYR, TRP-1和TRP-2基因水平的表达,(3)显著减少TRP-2的蛋白质表达。因此,红枫GCGs的抗黑色素原特性使我们得以对该自然产物的提取物进行更进一步的在美妆应用方面的研究。





Abstract 

     The red maple (Acer rubrum) is a rich source of phenolic compounds which possess galloyl groups attached to different positions of a 1,5-anhydro- d -glucitol core. While these glucitol-core containing gallotannins (GCGs) have reported anti-oxidant and anti-glycative effects, they have not yet been evaluated for their cosmetic applications. Herein, the anti-tyrosinase and anti-melanogenic effects of a proprietary phenolic-enriched red maple leaves extract [Maplifa ™ ; contains ca. 45% ginnalin A (GA) along with other GCGs] were investigated using enzyme and cel- lular assays. The GCGs showed anti-tyrosinase activity with IC 50 values ranging from 101.4 to 1047.3 μM and their mechanism of tyrosinase inhibition (using GA as a representative GCG) was evaluated by chelating and computational/modeling studies. GA reduced melanin content in murine melanoma B16F10 cells by 79.1 and 56.7% (at non-toxic concentrations of 25 and 50 μM, respectively), and its mechanisms of anti-melanogenic effects were evaluated by using methods including fluorescent probe (DCF-DA), real-time PCR, and western blot experiments. These data indicated that GA was able to: (1) reduce the levels of reactive oxygen species, (2) down-regulate the expression of MITF, TYR, TRP-1, and TRP-2 gene levels in a time-dependent manner, and (3) significantly reduce protein expression of the TRP-2 gene. Therefore, the anti-melano-genic effects of red maple GCGs warrant further investigation of this proprietary natural product extract for potential cosmetic applications.




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