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关于眼霜中芽孢孔菌菌落检测的影响因素的研究

更新日期:2017年05月02日


FACTORS OF DETECTION OF BACILLUS CEREUS STRAINS IN EYE CREAM

关于眼霜中芽孢孔菌菌落检测的影响因素的研究


  ABSTRACT

 Bacillus cereus has been associated with clinical infections and is also the cause of posttraumatic endophthalmitis as well as endogenous eye infections, which can result in blindness.Cosmetics, although preserved, can be contaminated during manufacture or use, and thus cause serious health issues. 

  OBJECTIVE: We investigated the detection factors of Bacillus in eye cream preserved with parabens, including the nonionic surfactants used as neutralizers such as Tween 80, a blend of Tween 60 and Span 80, Tween 20 and selective media.

  METHODS: Eye cream samples were first mixed with neutralizers and individually inoculated with B. cereus strains, B. mycoides, B. subtilis or B. thuringiensis at a final concentration of 5 log CFU/g. The inoculated samples with and without neutralizers were analyzed after 30 min and during 84-day storage at room temperature. Presumptive colonies of Bacillus were enumerated on the varieties of Bacillus agar by spiral-plating techniques and Most Probable Number (MPN) method.

  RESULTS: The recovery counts of all Bacillus strains were between 4.10 and 4.58 log CFU/g in samples with Tween 80 and from 3.62 to 4.53 CFU/g in samples with TS after 30 min. Tween 20 was the least effective neutralizer. The challenged organisms, in samples without neutralizer, B. subtilis ATCC 15563 and B. cereus 4227A were detected at 1.83 and 1.49 log CFU/g after 30 min, respectively.

  CONCLUSION: This study showed that Tween 80 was the best neutralizer for reducing the antimicrobial effect of parabens. BACARA® and R&F plating media showed typical reaction of Bacillus cereus strains.




接要

  急性感染与芽孢杆菌的存在息息相关,同时芽孢杆菌也是外伤性眼内炎和内源性眼睛感染的原因之一,严重易导致失明。化妆品虽然含有防腐功能,但是在生产或是使用过程中也会被污染,这样也容易导致严重的健康问题。

  目标:这个文章主要是研究对羟基苯甲酸酯用于眼霜防腐中对芽孢杆菌的影响因子,如作为中性剂的非离子表面活性剂吐温80、吐温60和司班80的复合物,吐温20和其他可选择的媒介。

方法:眼霜样品先用中和试剂混匀,再分别接种5log CFU/g蜡样芽孢杆菌、蕈状芽孢杆菌、枯草芽孢杆菌、苏云金芽孢杆菌。加和没加中性试剂的接种样口都在30min后及室温下储放84天中进行分析。

  结果显示:培养30min中后检测,含有吐温80的样品,所有的芽孢杆菌菌落数都在4.10-4.58 log CFU/g之间;加了吐温60和司班80的复合物,样品中芽孢杆菌数从3.62 增加到了4.53 log CFU/g。吐温20是最无效的中性试剂。在挑战性实验中,没有中和试剂的样品,ATCC15563 枯草芽孢杆菌和 4227A蜡样芽孢杆菌培养30分钟后分别在检测到 1.83 log CFU/g和1.49 log CFU/g。

  结论:这个研究表明吐温80是降低对羟基苯甲酸酯抑菌作用最好的中性试剂。




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